dir1 = getDirectory("Choose Source Directory ");
 dir2 = getDirectory("Choose Destination Directory ");
 list = getFileList(dir1);
 setBatchMode(true);
 for (i=0; i<list.length; i++) {
    print((i+1)+"/"+list.length+": "+list[i]);
    open(dir1+list[i]);
    run("Subtract Background...", "rolling=50 stack");
    run("Auto Threshold", "method=Max entropy background=Dark calculate black");
    run("Z Project...", "projection=[Sum Slices] all");
    run("Divide...", "value=255 stack");
    run("Enhance Contrast", "saturated=0.35");
    run("Fire");
    run("Calibration Bar...",
      "location=[Upper Left] fill=None label=White number=7 font=9 zoom=1.5 bold overlay");
    saveAs("tif", dir2+list[i]);
    run("Close All");
 }