Manual Tracking
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Author: |
Fabrice Cordelires, Institut Curie, Orsay
(France). fabrice.cordelieres
at curie.u-psud.fr |
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History: |
2004/06/25:
First version |
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Source: |
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Installation: |
Download
Manual_Tracking.class
to the plugins folder and restart ImageJ. |
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Description: |
This
plug-in allows the user to quantify movement of objects between frames of a
temporal stack. Results
table: This
plug-in provides a way to retrieve in a table (figure 1) X and Y coordinates
as well as velocity, distance covered between two frames and intensity of the
selected pixel, by simply clicking on the structure of interest. Figure 1:
Note: as
the first velocity value can't be calculated (first tracked frame where time
interval equals zero), its first displayed value will be -1, as in most of
the commercial software. Interface: Figure 2:
The
interface is composed of 3 parts: á
Calibration
values: this
section contains the time interval box where the user has to enter the time
delay between two consecutive images, and the distance calibration box where
user has to enter the size corresponding to one pixel. á
Tracking
buttons: o
To
start recording one track, the user has to click on "add track". o
The
tracking is done by clicking on the structure on the image. After each mouse
click, the following image of the temporal stack is activated until the last
image is reached or the "End track" button is pressed. o
"Delete
last point" is used to erase the last recorded coordinates. o
"End
track" button should be used to stop the tracking procedure in case the
structure disappeared from the image. o
By
selecting the number in the list next to the "Delete track n¡"
button, the user is allowed to erase one track from the result table. This
choice should be validated by clicking on the "Delete track n¡"
button. o
"Delete
all tracks" is used to clean up the results table. á
Display: this section deals with the ways
to get a visual representation of the coordinates recorded in the results
table.. Two drawing options can be defined in the corresponding sections of
the interface: the dot size and the line width. Several displays are
available: each button's function is illustrated in the following section Display: The
Manual Tracking module also have display capacities aiming to provide either
a synthetic vision of the tracked points and/or their paths (figure 3), or an
overlay of one of the synthetic representations and the original image
(figure 4). Figure 3:
Figure 4:
Images
Courtesy of Hlne Rangone, Institut Curie, Orsay (France). |
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