TTorg (Transverse tubular system regularity analysis)

Authors: Côme PASQUALIN (come.pasqualin at univ-tours dot fr) - François GANNIER (gannier at univ-tours dot fr)
History: 2014/09/03: first version
Source: see IJM file inside Jar.
Requires: ImageJ 1.47 or above.
Installation: The latest version of this plugin is available from pccv.univ-tours.fr/ImageJ/TTorg/.
Description: The TTorg plugin uses microscopy images (usually opened with the Bio-formats plugin) to analyse the organization level and the regularity of the transverse tubular system in muscle cells. See article for a complete description :
"AUTOMATIC QUANTITATIVE ANALYSIS OF T-TUBULE ORGANIZATION IN CARDIAC MYOCYTES USING IMAGEJ"
Côme Pasqualin, François Gannier, Claire O Malecot, Pierre Bredeloux, Véronique Maupoil
American Journal of Physiology - Cell Physiology 2014,
DOI: 10.1152/ajpcell.00259.2014

TTorg interface uses a minimum set of options to reduce human intervention. Minimum and maximum spacing are low and high pass filters. A threshold can be set (SSPD) to adjust the sensitivity and specificity of transverse tubules detection.

[interface] [interface2]

Menu and output for organization analysis.

[FFT]

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